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The slides were washed in PBS and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer

A study revealed that L-methionine supplementation (1.7 g/kg/day, P.O for 8 weeks) was sufficient to produce hyperhomocysteinemia in rats and markedly increased the mean arterial pressure, oxidative stress and mean cardiomyocyte diameter [107]